DNA

Part:BBa_K2066504:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-08-25)


ICA:BsmBI Cut Site + G + Sticky End 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

G was selected as the spacer nucleotide as per Briggs et al., 2012. Sticky End 2 was chosen as per supplemental material from Briggs et al., 2012.


Source

This part was synthesized as part of a gBlock / using IDT DNA synthesis. Part sequence based off of supplemental material from Briggs et al., 2012.

References